SubtiBank SubtiBank
ykoT [2018-02-12T00:18:55.000Z]
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ykoT [2018-02-12T00:18:55.000Z]

similar to lipoteichoic acid glycosyltransferase
locus
BSU13390
pI
9.34
mw
38.36 kDa
protein length
337 aa Sequence Blast
gene length
1011 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.5|Newly identified sporulation proteins (based on transcription profiling)]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

    • Gene

    Coordinates
    1,403,479 → 1,404,492

    The protein

    Protein family

  • glycosyltransferase 2 family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|FD76A781162795EAF174B59C0A90D2ED3ACC0AB7|YkcC] (39%), [protein|824E6AE2FFA3CBC9F90648F15178A00A6CC49E4C|CsbB] (31,1%)

    • Structure

  • [PDB|5EKP] (from Synechocystis sp., 38% identity) [pubmed|26729507]

    • [SW|Localization]

  • cell membrane (heterogeneous) [Pubmed|16479537]

    • Expression and Regulation

    Operons

    genes
    [gene|6B66E6E138ED70318E5911F310953D8F09FA8719|ykoS]-[gene|EF18470AD2B07755431534B52A8FCE6754FDBA9D|ykoT]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|26577401], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

    • regulatory mechanism

  • [protein|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|SpoVT]: repression, [Pubmed|26577401], in [regulon|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|SpoVT regulon]

    • regulation

  • expressed late during [SW|sporulation] in the forespore ([SW|SigG], [SW|SpoVT]) [Pubmed|26577401]
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    Biological materials

    Mutant

  • MGNA-A777 (ykoT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/777 NBRP B. subtilis, Japan]
  • BKE13390 (Δ[gene|EF18470AD2B07755431534B52A8FCE6754FDBA9D|ykoT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGACTGTTTCAATCGATATC, downstream forward: _UP4_TAAACACGGAAAGAGCTGAC
  • BKK13390 (Δ[gene|EF18470AD2B07755431534B52A8FCE6754FDBA9D|ykoT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGACTGTTTCAATCGATATC, downstream forward: _UP4_TAAACACGGAAAGAGCTGAC

    • References

  • 21630458,16479537,22383849,26577401,27185829,26729507